Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution. The presented techniques include powerful tools with a long tradition in RNA research as well as more advanced, novel methods, thus the methods span multiple disciplines, including molecular biology, biochemistry, biophysics, and computational biology.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Folding: Methods and Protocols serves as a vital reference for researchers attempting to gain insights into the secrets of this astounding macromolecule.

Inhaltsangabe1. Predicting RNA Structure: Advances and Limitations            Ivo L. Hofacker and Ronny Lorenz 2. Cis-Acting 5' Hammerhead Optimization for In Vitro Transcription of Highly Structured RNAs            Mélanie Meyer and Benoît Masquida 3. RNA Structural Analysis by Enzymatic Digestion            Elisa Biondi and Donald H. Burke 4. A Mutate-and-Map Protocol for Inferring Base Pairs in Structured RNA            Pablo Cordero, Wipapat Kladwang, Christopher C. VanLang, and Rhiju Das 5. Mapping RNA Structure In Vitro Using Nucleobase-Specific Probes            Nora Sachsenmaier, Stefan Handl, Franka Debeljak, and Christina Waldsich 6. Massively Parallel RNA Chemical Mapping with a Reduced Bias MAP-Seq Protocol            Matthew Seetin, Wipapat Kladwang, John. P. Bida, and Rhiju Das 7. Probing RNA Folding by Hydroxyl Radical Footprinting            Maria Costa and Dario Monachello 8. Monitoring Global Structural Changes and Specific Metal Binding Sites in RNA by In-Line Probing and Tb(III) Cleavage            Pallavi K. Choudhary, Sofia Gallo, and Roland K.O. Sigel 9. Chemical Probing of RNA in Living Cells            Michael Wildauer, Georgeta Zemora, Andreas Liebeg, Verena Heisig, and Christina Waldsich 10. A Chemogenetic Approach to Study the Structural Basis of Protein-Facilitated RNA Folding            Olga Fedorova 11. Applying UV Crosslinking to Study RNA-Protein Interactions in Multicomponent Ribonucleoprotein Complexes            Andrey S. Krasilnikov 12. The Kinetics of Ribozyme Cleavage: A Tool to Analyze RNA Folding as a Function of Catalysis            Nora Zingler 13. RNA Catalytic Activity as a Probe of Chaperone-Mediated RNA Folding            Brant Gracia and Rick Russell 14. Evaluation of RNA Chaperone Activity In Vivo and In Vitro Using Misfolded Group I Ribozymes            Katharina Semrad 15. RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis            Sébastien H. Eschbach and Daniel A. Lafontaine 16. Detecting RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation            Somdeb Mitra 17. RNA Folding Dynamics Using Laser Assisted Single-Molecule Refolding            Bishnu Paudel and David Rueda 18. RNA Refolding Studied by Light-Coupled NMR Spectroscopy            Harald Schwalbe and Boris Fürtig 19. Exploring RNA Oligomerization and Ligand Binding by Fluorescence Correlation Spectroscopy and Small Angle X-Ray Scattering            Eileen Magbanua, Petr V. Konarev, Dmitri I. Svergun, and Ulrich Hahn

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