This well-organized text gives a concise account of various enzymes like protease & keratinase & aims to put the current knowledge into perspective. Proteases are a unique class of enzymes, since they have of immense physiological & commercial importance. They occur ubiquitously in animals, plants & microbes. However, microbes are a goldmine of proteases & represent the preferred source of enzymes by virtue of their rapid growth, limited space required for cultivation & ready accessibility to genetic manipulation. Microbial proteases have been extensively used in the food, dairy & detergent industries since ancient times. The development of recombinant rennin & its commercialization is an excellent example of the successful application of modern biology to biotech. This text discusses the behavior of isolated enzymes- protease & keratinase, dealing in turn with isolation, characterization & purification methods from isolated micro-organisms of soil sample of poultry waste site & various potential industrial applications of enzymes. These methods for isolation, characterization & purification of enzymes are necessary to know for its commercialization at large scale in industry.

Dr.Sarita Agrahari did her PhD in Biotech. from JIIT,Noida,India.She did her UG and PG from Amravati University,India.She has attended many international,national conferences and presented her research work both in India and abroad.She has been awarded travel grant by DBT, Govt.of India.Currently she is focusing on microbial and enzyme technology.

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