Beschreibung
InhaltsangabePart I: Cloning and Heterologous Expression of Ion Channel Genes 1. Approaches to Cloning of Pain-Related Ion Channel Genes Armen N. Akopian 2. Mammalian Expression Systems and Transfection Techniques Daunia Laurenti and Lezanne Ooi 3. Use of Escherichia coli for the Production and Purification of Membrane Proteins Vincent G.L. Postis, Andrea E. Rawlings, Amelia Lesiuk, and Stephen A. Baldwin 4. Transient Overexpression of Genes in Neurons Using Nucleofection Hannah M. Kirton, Louisa Pettinger, and Nikita Gamper 5. Viral Gene Delivery: Optimized Protocol for Production of High Titre Lentiviral Vectors James Hewinson, Julian F.R. Paton, and Sergey Kasparov Part II: Electrophysiological Methods to Study Ion Channel Functions 6. Two-Electrode Voltage Clamp Bingcai Guan, Xingjuan Chen, and Hailin Zhang 7. Conventional Micropipette-Based Patch Clamp Techniques Jonathan D. Lippiat and David C. Wrighton 8. Recording of Ion Channel Activity in Planar Lipid Bilayers Experiments Eleonora Zakharian 9. Recording Macroscopic Currents in Large Patches from Xenopus Oocytes Tibor Rohacs 10. Combined Single-Channel and Macroscopic Recording Techniques to Analyze Gating Mechanisms of the Large Conductance Ca2+ and Voltage Activated (BK) Potassium Channel Nguyen V. Nguyen, Aleksandra Gruslova, Wojciech A. Kosiba, and Bin Wang 11. Perforated Whole Cell Patch Clamp Recording John E. Linley 12. Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons Jizhe Hao, Jérôme Ruel, Bertrand Coste, Yann Roudaut, Marcel Crest, and Patrick Delmas 13. Automated Planar Patch-Clamp Carol J. Milligan and Clemens Möller 14. Recording Single-Channel Currents Using "Smart Patch-Clamp" Technique Anamika Bhargava and Julia Gorelik Part III: Imaging and Fluorescence Methods to Study Ion Channels 15. Using Total Internal Reflection Fluorescence Microscopy to Observe Ion channel Trafficking and Assembly Sarah Schwarzer, Gregory I. Mashanov, Justin E. Molloy, and Andrew Tinker 16. Förster Resonance Energy Transfer-Based Imaging at the Cell Surface of Live Cells Sonya M. Bierbower and Mark S. Shapiro 17. The Use of Dansyl-Calmodulin to Study Interactions with Channels and Other Proteins Alessandro Alaimo, Covadonga Malo, Pilar Areso, Kerman Aloria, Oscar Millet, and Alvaro Villarroel 18. Imaging and Quantification of Recycled KATP Channels Christopher J. Cockcroft Part IV: Biochemical and Structure-Functional Approaches in Ion Channel Studies 19. Generation of Antibodies that Are Externally-Acting Isoform-Specific Inhibitors of Ion Channels Jacqueline Naylor and David J. Beech 20. Site-Directed Mutagenesis to Study the Structure-Function Relationships of Ion Channels Wei Yang and Lin-Hua Jiang 21. Cysteine-Based Cross-Linking Approach to Study Inter-Domain Interactions in Ion Channel Functions Lin-Hua Jiang 22. Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-Ray Crystallography Frank J. Smith and Brad S. Rothberg 23. Isotope Labeling Strategies for Analysis of an Ion Channel Cytoplasmic Domain by NMR Spectroscopy Karin Abarca-Heidemann, Elke Duchardt-Ferner, Jens Woehnert, and Brad S. Rothberg Part V: Studying Ion Channels in Native Tissue
Autorenportrait
InhaltsangabePart I: Cloning and Heterologous Expression of Ion Channel Genes 1. Approaches to Cloning of Pain-Related Ion Channel Genes Armen N. Akopian 2. Mammalian Expression Systems and Transfection Techniques Daunia Laurenti and Lezanne Ooi 3. Use of Escherichia coli for the Production and Purification of Membrane Proteins Vincent G.L. Postis, Andrea E. Rawlings, Amelia Lesiuk, and Stephen A. Baldwin 4. Transient Overexpression of Genes in Neurons Using Nucleofection Hannah M. Kirton, Louisa Pettinger, and Nikita Gamper 5. Viral Gene Delivery: Optimized Protocol for Production of High Titre Lentiviral Vectors James Hewinson, Julian F.R. Paton, and Sergey Kasparov Part II: Electrophysiological Methods to Study Ion Channel Functions 6. Two-Electrode Voltage Clamp Bingcai Guan, Xingjuan Chen, and Hailin Zhang 7. Conventional Micropipette-Based Patch Clamp Techniques Jonathan D. Lippiat and David C. Wrighton 8. Recording of Ion Channel Activity in Planar Lipid Bilayers Experiments Eleonora Zakharian 9. Recording Macroscopic Currents in Large Patches from Xenopus Oocytes Tibor Rohacs 10. Combined Single-Channel and Macroscopic Recording Techniques to Analyze Gating Mechanisms of the Large Conductance Ca2+ and Voltage Activated (BK) Potassium Channel Nguyen V. Nguyen, Aleksandra Gruslova, Wojciech A. Kosiba, and Bin Wang 11. Perforated Whole Cell Patch Clamp Recording John E. Linley 12. Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons Jizhe Hao, Jérôme Ruel, Bertrand Coste, Yann Roudaut, Marcel Crest, and Patrick Delmas 13. Automated Planar Patch-Clamp Carol J. Milligan and Clemens Möller 14. Recording Single-Channel Currents Using "Smart Patch-Clamp" Technique Anamika Bhargava and Julia Gorelik Part III: Imaging and Fluorescence Methods to Study Ion Channels 15. Using Total Internal Reflection Fluorescence Microscopy to Observe Ion channel Trafficking and Assembly Sarah Schwarzer, Gregory I. Mashanov, Justin E. Molloy, and Andrew Tinker 16. Förster Resonance Energy Transfer-Based Imaging at the Cell Surface of Live Cells Sonya M. Bierbower and Mark S. Shapiro 17. The Use of Dansyl-Calmodulin to Study Interactions with Channels and Other Proteins Alessandro Alaimo, Covadonga Malo, Pilar Areso, Kerman Aloria, Oscar Millet, and Alvaro Villarroel 18. Imaging and Quantification of Recycled KATP Channels Christopher J. Cockcroft Part IV: Biochemical and Structure-Functional Approaches in Ion Channel Studies 19. Generation of Antibodies that Are Externally-Acting Isoform-Specific Inhibitors of Ion Channels Jacqueline Naylor and David J. Beech 20. Site-Directed Mutagenesis to Study the Structure-Function Relationships of Ion Channels Wei Yang and Lin-Hua Jiang 21. Cysteine-Based Cross-Linking Approach to Study Inter-Domain Interactions in Ion Channel Functions Lin-Hua Jiang 22. Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-Ray Crystallography Frank J. Smith and Brad S. Rothberg 23. Isotope Labeling Strategies for Analysis of an Ion Channel Cytoplasmic Domain by NMR Spectroscopy Karin Abarca-Heidemann, Elke Duchardt-Ferner, Jens Woehnert, and Brad S. Rothberg Part V: Studying Ion Channels in Native Tissue
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