Thousands of proteins have been identified to be acetylated. Immense research power has been dedicated to experiments to solve the biological implications of each and every protein acetylation. Two particular sites of protein acetylation have been described intensively: the N-terminal methionine residue of a nascent protein and lysine residues within a protein. In Protein Acetylation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study protein acetylation. These include methods and techniques for identification of protein acetylation, column- and gel electrophoresis-based approaches, computationally prediction,  and the biological response to protein acetylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Acetylation: Methods and Protocols seeks to aid scientists in the further study of the technical aspects involved in understanding protein acetylation.

Inhaltsangabe1. Validation of Protein Acetylation by Mass SpectrometryBarry M. Zee and Benjamin A. Garcia2. Application of the CIRAD Mass Spectrometry Apprach for Lysine Acetylation Site DiscoveryCaroline A Evans, Saw Yen Ow, Duncan L Smith, Bernard M Corfe, and Phillip C Wright3. Application of the MIDAS Approach for Analysis of Lysine Acetylation SitesCaroline A Evans, John R Griffiths, Richard D Unwin, Anthony D Whetton, Bernard M Corfe4. Application of High Content Biology to Yield Quantitative Spatial Proteomic Information on Protein AcetylationsBernard M Corfe, Josephine Kilner, Joanna Chowdry, Roderick SP Benson, Gareth J Griffiths,  Caroline A Evans5. Towards the N-terminal Acetylome: An N-terminal Acetylated Peptide Enrichment Method Using CNBr-Activated Sepharose ResinXumin Zhang and Peter Højrup 6. Identification and Analysis of O-acetylated SialoglycoproteinsChandan Mandal and Chitra Mandal 7. HPLC-based Quantification of in vitro N-terminal AcetylationRune H. Evjenth, Petra Van Damme, Kris Gevaert, Thomas Arnesen8. Separation and Purification of Multiply Acetylated Proteins using Cation-exchange ChromatographyRomeo Papazyan and Sean D. Taverna 9. In-gel N-acetylation for the Quantification of the Degree of Protein in vivo N-terminal AcetylationPetra Van Damme, Thomas Arnesen, Bart Ruttens, Kris Gevaert 10. Computational Prediction of Lysine Acetylation Proteome-wide Amrita Basu11. Generation and Characterization of Pan-specific Anti-acetyllysine AntibodyDr. Wei Xu and Dr. Shimin Zhao12. Using Functional Proteome Microarrarys to Study Protein Lysine AcetylationJin-Ying Lu, Yu-yi Lin, Jef D. Boeke, and Heng Zhu13. Quantitation of Nucleosome Acteylation and other Histone Post-Translational Using Microscale NU-ELISABo Dai, Charles Giardina, Theodore P. Rasmussen14. Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome CoreJohn C. Shimko, Cecil J. Howard, Michael G. Poirier, and Jennifer J. Ottesen 15. Production of Amino-terminally Acetylated Recombinant Proteins in E. coliMatthew Johnson, Michael A. Geeves and Daniel P. Mulvihill 16. Identification of Lysine Acetyltransferase Substrates using Bioorthogonal Chemical ProteomicsMarkus Grammel and Howard C. Hang17. Non-radioactive in-vitro Assays for Histone DeacetylasesAlexander-Thomas Hauser, Julia M. Gajer (née Wagner), and Manfred Jung 18. The Fluorescence-Based Acetylation Assay Using Thiol-Sensitive ProbesTielong Gao, Chao Yang, Yujun George Zheng19. Analysis of Protein Acetyltransferase Structure-function Relation by Surface-Enhanced Raman Scattering (SERS): A Tool to Screen and Characterize Small Molecule ModulatorsMohammed Arif, Dhanasekaran Karthigeyan, Soumik Siddhanta, G. V. Pavan Kumar, Chandrabhas Narayana, and Tapas K. Kundu