In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world.

Inhaltsangabe1. A Bird's Eye View of the Bacterial Landscape            Natividad Ruiz 2. Visualizing the Bacterial Cell Surface: An Overview            Harald Engelhardt 3. Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus Systems            Nadine S. Henderson and David G. Thanassi 4. Experimental Manipulation of the Microbial Functional Amyloid Called Curli            Yizhou Zhou, Daniel R. Smith, David A. Hufnagel, and Matthew R. Chapman 5. Visualization of Gram-Positive Bacterial Pili            Chungyu Chang, I-Hsiu Huang, Antoni P. Hendrickx, and Hung Ton-That 6. Single Cell Microfluidic Studies of Bacterial Motility            Adrien Ducret, Olivier Théodoly, and Tâm Mignot 7. Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule            Susan M. Steenbergen and Eric R. Vimr 8. Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning Microscopy            Wei Hu, Renate Lux, and Wenyuan Shi 9. Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance            Elena B. Tikhonova and Helen I. Zgurskaya 10. Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion System            Tanya L. Johnson, Aleksandra E. Sikora, Ryszard A. Zielke, and Maria Sandkvist 11. Pore Formation by T3SS Translocators: Liposome Leakage Assay            Eric Faudry, Caroline Perdu, and Ina Attrée 12. Isolation of Bacterial Type IV Machine Subassemblies            Mayukh K. Sarkar, Seyyed I. Husnain, Simon J. Jakubowski, and Peter J. Christie 13. Production and Crystallization of Bacterial Type V Secretion Proteins            Hye-Jeong Yeo 14. Assembly of Bacterial Outer Membrane Proteins            Jan Grijpstra, Martine P. Bos, and Jan Tommassen 15. The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and Characterization            Jessica V. Hankins, James A. Madsen, Brittany D. Needham, Jennifer S. Brodbelt, and M. Stephen Trent 16. Quantitative and Qualitative Preparations of Bacterial Outer Membrane Vesicles            Halima Chutkan, Ian MacDonald, Andrew Manning, and Meta J. Kuehn 17. In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate            Jacob Biboy, Nhat Khai Bui, and Waldemar Vollmer 18. Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes            Filipe Carvalho, María Graciela Pucciarelli, Francisco García-del Portillo, Didier Cabanes, and Pascale Cossart 19. Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis            Anna E. Grzegorzewicz and Mary Jackson 20. Protein Disulfide Bond Formation in the Periplasm: Determination of the In Vivo Redox State of Cysteine Residues            Katleen Denoncin, Valerie Nicolaes, Seung-Hyun Cho, Pauline Leverrier, and Jean-Francois Collet 21. Using Reporter Genes and the Escherichia coli ASKA Over-Expression Library in Screens for Regulators of the Gram Negative Envelope Stress Response            Julia L. Wong, Stefanie L. Vogt, and Tracy L. Raivio 22. Isolation of Bacteria Envelope Proteins            Shu Quan, Annie Hiniker, Jean-Francois Collet, and James C.A. Bardwell 23. Pa

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