Understanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease.  In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biologya''¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and easy-to-use, Gene Expression Profiling: Methods and Protocols, Second Edition presents a collection of clearly described and illustrated chapters, certain to be helpful to researchers in academia, in hospitals, and in industry who are interested in applying techniques, whether basic or advanced, for the analysis of gene expression.

1. Real-Time PCR and Multiplex Approaches             Olga L. Gurvich and Mikhail Skoblov   2. Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs             Sweta Rani and Lorraine O¿Driscoll   3. Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals             Jai Prakash Mehta   4. Software and Tools for Microarray Data Analysis             Jai Prakash Mehta and Sweta Rani   5. Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumor Cells             Anne M. Friel, John Crown, and Lorraine O¿Driscoll   6. Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay             Craig S. April and Jian-Bing Fan   7. MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs             Erica Hennessy and Lorraine O¿Driscoll   8. Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases             Serena Germano and Lorraine O¿Driscoll   9. 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins             Paula Meleady   10. Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis             Kathy Gately, Keith Kerr, and Ken O¿Byrne   11. Immunohistochemical and Immunofluorescence Procedures for Protein Analysis             Kishore Reddy Katikireddy and Finbarr O¿Sullivan   12. Advanced Microscopy: Laser Scanning Confocal Microscopy             Orla Hanrahan, James Harris, and Chris Egan   13. Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling             Sweta Rani, Keith O¿Brien, Fergal C. Kelleher, Claire Corcoran, Serena Germano, Marek Radomski, John Crown, and Lorraine O¿Driscoll   14. Atomic Force Microscopy (AFM) and High-Content Analysis (HCA): Two Innovative Technologies for Dissecting the Relationship Between Epithelial-Mesenchymal Transition (EMT)-Related Morphological and Structural Alterations and Cell Mechanical Properties             Stephen T. Buckley, Anthony M. Davies, and Carsten Ehrhardt