Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.

Inhaltsangabe1. Photoinduced Damage Resulting from Fluorescence Imaging of Live Cells Matthew K. Daddysman, Michael A. Tycon, and Christopher J. Fecko 2. Modification of Purified Proteins with Photochemical Protection Compounds for High-Resolution Photoactivation of Protein Function In Vitro and In VivoSidney B. Cambridge 3. Optochemical Activation of Kinase Function in Live Cells Andrei V. Karginov, Klaus M. Hahn, and Alexander Deiters 4. Photoswitching of Cell Surface Receptors Using Tethered LigandsAndreas Reiner and Ehud Y. Isacoff 5. Photocontrol of AMPA Receptors with a Photochromic LigandMartin Sumser and Philipp Stawski 6. Photoconversion of CFP to Study Neuronal Tissue with Electron MicroscopyNina Wittenmayer 7. Light-Inducible Gene Regulation with Engineered Zinc Finger ProteinsLauren R. Polstein and Charles A. Gersbach 8. Manipulation of Plasma Membrane Phosphoinositides using Photoinduced Protein-protein InteractionsOlof Idevall-Hagren and Pietro DeCamilli 9. A Roadmap to Applying Optogenetics in NeuroscienceConsuelo Fois, Pierre-Hugues Prouvot, and Albrecht Stroh 10. Salvaging Ruins: Reverting Blind Retinas into Functional Visual SensorsMarion Mutter, Natalia Swietek & Thomas A. Münch 11. Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal ActivityWagner Steuer Costa, Jana Liewald, Alexander Gottschalk 12. Structural Basis of Photoswitching in Fluorescent ProteinsChenxi Duan, Virgile Adam, Martin Byrdin and Dominique Bourgeois 13. Using Photoactivatable GFP to Track Axonal Transport KineticsArchan Ganguly and Subhojit Roy 14. In Vivo Cell Tracking Using PhOTO ZebrafishWilliam P. Dempsey, Hanyu Qin, Periklis Pantazis 15. In vivo Optogenetics for Light-induced Oxidative Stress in Transgenic Zebrafish  Expressing the KillerRed Photosensitizer ProteinCathleen Teh and Vladimir Korzh 16. Photoactivatable Fluorescent Proteins for Super-Resolution MicroscopyYuji Ishitsuka, Karin Nienhaus, and G. Ulrich Nienhaus 17. pcSOFI as a Smart Label-based Super resolution Microscopy TechniqueBenjamien Moeyaert and Peter Dedecker

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