The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and Protocols provides a detailed description of the currently available methods and protocols to investigate bacterial and fungal biofilms, exhaustively illustrated and critically annotated in 25 chapters written by authors well known for their experience in the respective fields. The book has joined together microbiologists and specialists in infectious diseases, hygiene and public health involved in exploring different aspects of microbial biofilms as well as in designing new methods and/or developing innovative laboratory protocols. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Microbial Biofilms: Methods and Protocols presents readers with the most established and validated experimental procedures to investigate microbial biofilms.

InhaltsangabePart I. Investigations on Biofilms in Health and Disease 1. Methods for Dynamic Investigations of Surface Attached in vitro Bacterial and Fungal BiofilmsClaus Sternberg, Thomas Bjarnsholt, and Mark Shirtliff 2. Aqueous Two-Phase System Technology for Patterning Bacterial Communities and Biofilms Mohammed Dwidar, Shuichi Takayama, and Robert J. Mitchell 3. Quorum Sensing in Gram-Positive Bacteria: Assay Protocols for Staphylococcal agr and enterococcal fsr SystemsAkane Shojima and Jiro Nakayama 4. Advanced Techniques for in situ Analysis of the Biofilm Matrix (Structure, Composition, Dynamics) by Means of Laser Scanning Microscopy    Thomas R. Neu and John R. Lawrence 5. Multiplex Fluorescence in situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) to Analyse Multispecies Oral Biofilms Lamprini Karygianni, Elmar Hellwig, Ali Al-Ahmad  6. Field Emission Scanning Electron Microscopy of Biofilm-Growing Bacteria Involved in Nosocomial InfectionsClaudia Vuotto and Gianfranco Donelli 7. Experimental Approaches to Investigating the Vaginal Biofilm MicrobiomeMarc M. Baum, Manjula Gunawardana, and Paul Webster 8. Imaging Bacteria and Biofilms on Hardware and Periprosthetic Tissue in Orthopaedic InfectionsL. Nistico,  L. Hall-Stoodley, and P. Stoodley 9. Animal Models to Evaluate Bacterial Biofilm DevelopmentKim Thomsen, Hannah Trøstrup, and Claus Moser 10. Animal Models to Investigate Fungal Biofilm FormationJyotsna Chandra, Eric Pearlman, and Mahmoud A. Ghannoum 11. Non-Mammalian Model Systems to Investigate Fungal BiofilmsMarios Arvanitis, Beth Burgwyn Fuchs, and Eleftherios Mylonakis Part II. Investigations on anti-biofilm compounds and strategies  12. Microbiological Methods for Target-Oriented Screening of Biofilm InhibitorsLivia Leoni and Paolo Landini 13. In vitro Screening of Antifungal Compounds Able to Counteract Biofilm DevelopmentMarion Girardot and Christine Imbert 14. Biofilm Matrix-Degrading EnzymesJeffrey B. Kaplan 15. Efficacy Evaluation of  Antimicrobial Drug-Releasing Polymer Matrices Iolanda Francolini, Antonella Piozzi, and Gianfranco Donelli 16. Antibiotic Polymeric Nanoparticles for Biofilm-Associated Infection TherapyWean Sin Cheow and Kunn Hadinoto  17. Pharmacokinetics and Pharmacodynamics of Antibiotics in Biofilm Infections of Pseudomonas aeruginosa in vitro and in vivo Wang Hengzhuang, Niels Høiby, and Oana Ciofu 18. Contribution of Confocal Laser Scanning Microscopy in Deciphering Biofilm Tridimensional Structure and Reactivity   Arnaud Bridier and Romain Briandet 19. Chip-Calorimetry for Evaluation of Biofilm Treatment with Biocides, Antibiotics and Biological AgentsFrida Mariana, Friederike Buchholz, and Thomas Maskow  20. Bacteriophage Attack as an Anti-Biofilm StrategySanna Sillankorva and Joana Azeredo 21. Photodynamic Therapy as a Novel Antimicrobial Strategy Against Biofilm-Based Nosocomial Infections: Study ProtocolsFrancesco Giuliani Part III. Investigations on biofilms in the environment and manufacturing plants 22. Capturing Air-Water Interface Biofilms for Microscopy and Molecular AnalysisMargaret C. Henk 23. Biofilm-Growing Bacteria Involved in the Corrosion of Concrete Wastewater Pipes: Protocols for Comparative Metagenomic AnalysesVicente Gomez-Alvarez 24. Culture-Independent Methods to Study Subaerial Biofilm Growing on Biodeteriorated Surfaces of Cultural Heritage Stone Materials and FrescoesFrancesca Cappitelli, Federica Villa, and Andrea Polo<

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