Providing a selection of the key techniques that are used in characterizing cerebral angiogenesis, Cerebral Angiogenesis: Methods and Protocols aims to define the cellular and molecular mechanisms underlying this important process. Divided into six parts, this detailed volume examines cerebral angiogenesis occurring in different scenarios, a variety of different models in which cerebral angiogenesis can be studied, methods to characterize and quantify angiogenic events as well as several different approaches to measure changes in cerebral blood flow, different approaches to investigate the role of specific candidate genes in cerebral angiogenesis, methods of therapeutically manipulating cerebral angiogenesis by gene delivery, and finally important approaches to examine cerebral angiogenic mechanisms in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and decidedly practical, Cerebral Angiogenesis: Methods and Protocols should be a vital resource for all researchers, both those new to this field as well as those looking to use more specialized and sophisticated techniques to examine blood vessel growth in the brain.

InhaltsangabePart I: Overview of Cerebral Angiogenesis 1. Cerebral Angiogenesis During Development: Who's Conducting the Orchestra?            Ina M. Wittko-Schneider, Fabian T. Schneider, and Karl H. Plate 2. Cerebral Angiogenesis: A Realistic Therapy for Ischemic Disease?            David A. Greenberg 3. Vascular Normalization in Cerebral Angiogenesis: Friend or Foe?            Jisook Lee, Andrew Baird, and Brian P. Eliceiri 4. Pericytes and Adaptive Angioplasticity: The Role of Tumor Necrosis Factor Weak Inducer of Apoptosis (TWEAK)            Paula Dore-Duffy Part II: Animal Models of Cerebral Angiogenesis 5. Analysis of Angiogenesis in the Developing Mouse Central Nervous System            Nicole Ziegler, Karl H. Plate, and Stefan Liebner 6. Hypoxia-Induced Angiogenesis and Capillary Density Determination            Constantinos P. Tsipis, Xiaoyan Sun, Kui Xu, and Joseph C. LaManna 7. The Middle Cerebral Artery Occlusion Model of Transient Focal Cerebral Ischemia            Fudong Liu and Louise D. McCullough 8. A Mouse Model of Chronic Cerebral Hypoperfusion Characterizing Features of Vascular Cognitive Impairment            Masafumi Ihara, Akihiko Taguchi, Takakuni Maki, Kazuo Washida, and Hidekazu Tomimoto 9. A Mouse Model of Permanent Focal Ischemia: Distal Middle Cerebral Artery Occlusion            Kristian P. Doyle and Marion S. Buckwalter 10. A Method of Inducing Global Cerebral Ischemia            Gina Hadley, Michalis Papadakis, and Alastair M. Buchan 11. Induction of Cerebral Arteriogenesis in Mice            André Duelsner, Nora Gatzke, Anja Bondke Persson, and Ivo R. Buschmann 12. Vessel Painting Technique for Visualizing the Cerebral Vascular Architecture of the Mouse            Shea Hughes, Olkesankr Dashkin, and Richard Anthony DeFazio 13. Examining Cerebral Angiogenesis in Response to Physical Exercise            Kiersten L. Berggren, Jacob J.M. Kay, and Rodney A. Swain Part III: Methods of Examining Cerebral Angiogenesis 14. Histological Assessment of Angiogenesis in the Hypoxic Central Nervous System            Moises Freitas-Andrade, Jacqueline Slinn, Claudie Charlebois, and Maria J. Moreno 15. Examining Vascular Remodeling in the Hypoxic Central Nervous System            Amin Boroujerdi, Jennifer V. Welser-Alves, and Richard Milner 16. Analysis of Cerebral Angiogenesis in Human Glioblastomas            Michel Mittelbronn, Peter Baumgarten, Patrick N. Harter, and Karl H. Plate 17. Quantitative Cerebral Blood Flow Measurements Using MRI            Eric R. Muir, Lora Talley Watts, Yash Vardhan Tiwari, Andrew Bresnen, and Timothy Q. Duong 18. Fluorescent Angiogenesis Models Using Gelfoam® Implanted in Transgenic Mice Expressing Fluorescent Proteins            Robert M. Hoffman 19. Laser Speckle Contrast Imaging to Measure Changes in Cerebral Blood Flow            Ian R. Winship 20. Laser Doppler Flowmetry to Measure Changes in Cerebral Blood Flow            Brad A. Sutherland, Tamer Rabie, and Alastair M. Buchan Part IV: Determining the Role of Candidate Genes in Cerebral Angiogenesis 21. Defining the Role Of HIF and Its Downstream Mediators in Hypoxic-Induced Cerebral Angiogenesis            Xiaoyan Sun, Constantinos P. Tsipis, Girriso F. Benderro, Kui Xu, and Joseph C. LaManna 22. Inducible Gene Deletion in Glial Cells to Study Angiogenesis in the Central Nervous System            Hye Shin Lee and Joseph H. McCarty 23. Bone Marrow Chimera Experiments to Determine the Contribution of Hematopoiet